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Jack (John) N Saddler
Dean - Faculty of Forestry NSERC Industrial Senior Chair
Office of The Dean
Research Interests:
  • Application of enzymes in enhancing pulp and fibre properties
  • Fibre modification and bleach boosting pulps
  • Bioconversion of lignocellulosic residues to ethanol
  • Microbiology of waste water treatment
  • Application of fungi to upgrading and modification of forest products, pulp and paper and waste streams
    • Current Research:
  • Senior Chair in Forest Products Biotechnology - NSERC/Forintek/ Weyerhaeuser Canada
  • Substrate and enzyme factors that influence fiber modification - NSERC
  • Assessing factors that limit the enzymatic hydrolysis of softwood derived cellulose - NSERC
  • Bioconversion of softwoods to ethanol - NRC
  • Full characterization of western red cedar wood oil and the isolation and testing of tropolenes from the oil - BCASI/FRBC
  • Enzymatic and microbial treatment of pulp mill white waters - NCE
  • Use of immunoassays to monitor in-mill and waste water concentrations of hydrophobic, organic material - NCE
  • The use of enzymes to elucidate the structure and to modify native and recycled paper - NSERC/CFS/Industry
  • IEA bioconversion task, Biotechnology for the conversion of lignocellulosics- IEA
  • Life cycle analyses of biomass-to-ethanol - NCE

  • Supply of pretreated biomass for surface characterization and enzymatic digestion studies – NREL

  • Bioprocessing Centre for Sustainable Fuels - CFI

  • Development of a technically and economically viable pre-treatment and enzymatic process for the conversion of softwood residues to ethanol – NSERC/BIOCAP

  • Development of value-added bioproducts from the bioconversion of lignocellulosics – NSERC/BIOCAP

  • Development of biorefinery technologies for conversion of softwood residues to ethanol and co-products – NSERC

  • Participation in Biomass Consortium on Applied Fundamentals - NRC

    • Current grad students Graduate students

    Pooria Assadi, Supervisor - Chair - Academic and Research , Ph.D. started Monday, September 01, 2008
    Luis Del Rio, Supervisor - Chair - Academic and Research , Ph.D. started Saturday, September 01, 2007
  • Substrate characteristics that influence the cellulolytic hydrolysis of beetle-killed lodgepole pine organosolv pulps
    		•
    Michael Liu, Supervisor - Chair - Academic and Research , M.Sc. started Monday, January 01, 2007
  • Fermentation of hemicellulose-rich liquid fractions derived from steam-pretreated softwood.
    		•
    Linoj Kumar Naduvile Veettil, Supervisor - Chair - Academic and Research , M.Sc. started Tuesday, May 01, 2007
  • Steam pre-treatment and post-treatment of softwoods to enhance enzymatic hydrolysis and product recovery
    		•
    Seiji Nakagame, Supervisor - Chair - Academic and Research , Ph.D. started Saturday, September 01, 2007
  • The role of lignin in the hydrolysis of pretreated substrates by cellulases.
    		•
    Amadeus Pribowo, Supervisor - Chair - Academic and Research , M.Sc. started Monday, September 01, 2008
    Wenjuan Qin, Supervisor - Chair - Academic and Research , Ph.D. started Thursday, September 01, 2005
  • Potential application of high consistency enzymatic hydrolysis.
    		•
    Jamie Stephen, Supervisor - Chair - Academic and Research , Ph.D. started Thursday, May 01, 2008

    Awards:
    • LifeSciences BC Leadership Award, 2009
    • Fellow of the Royal Society of Canada, 2007
    • Advances in Science and Technology Award, US Dept. of Agriculture, Peoria Illinois, 2000
    • IUFRO Scientific Achievement 1999 (given out every 5 years)
    • Charles D. Scott Awards for Scientific/Technical Contributions to Biotechnology for the Production of Fuels and Chemicals, 1998

     

    Courses Taught:
    Links:
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    Additional Info:
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    Current publications

    Chandra, R.P., Ewanick, S.M., Hsseih, C. and Saddler, J.N. (2008) The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis part 1: A modified Somons' staining procedure -Botechnology Progress, 24(5):1178-1185
    Keating, J., Johansson, C.I., Saddler, J.N. and Beatson, R.P. (2008) Kinetics of hydrogen peroxide brightening of western red cedar (Thuja plicata Donn) CTMP -Journal of Wood Chemistry and Technology, 28(2):153-166
    Pan, X-J, Xie, D., Yu, R.W. and Saddler, J.N. (2008) The bioconversion of mountain pine beetle-killed lodgepole pine to fuel ethanol using the organosolv process -Biotechnology and Bioengineeering, 101(1):39-48
    Munoz, C., Menoca, R., Baeza, j., Berlin, A., Saddler, J.N. and Freer, J. (2007) Bioethanol production from bi-organosolv pulps of Pinus radiata and Acacia dealbata -Journal of Chemical Technology & Biotechnology, 82(8):767-774
    Saddler, J.N. (2007) The Biorefining Story: Progress in the commercialization of biomass-to-ethanol -Proceedings of the Bio Fuels World Conference Committee, July11-13, 2007, Yokohama, Japan
    Pan, X.J., Xie, D., Kang, Y., Yoon, S.L. and Saddler, J.N. (2007) Effect of organosolv ethanol pretreatment variables on physical characteristics of hybrid poplar substrates -Applied Biochemistry and Biotechnology 136-140:367-378
    Chandra, R.P., Bura, R., Mabee, W., Berlin, A., Pan, X. an Saddler, J.N. (2007) Substrate Pretreatment: The Key to Effective Enzymatic Hydrolysis of Lignocellulosics? -Special Volume of Advances in Biochemical Engineering/Biotechnology DOI 10.1007/10_2007_064
    Tu, Maobing, Chandra, R. and Saddler, J.N. (2007) Evaluating the Distribution of Cellulases and the Recycling of Free Cellulases during the Hydrolysis of Lignocellulosic Subtrates -Biotechnology Progress Publlication 23(2):398-406
    Chandra, R.P., Esteghalilan, A.R., and Saddler, J.N. (2007) Assessing Substrate Accessibility to Enzymatic Accessibility of Enzymatic Hydrolysis by Celulases -Book Chapter in American Chemical Society Book
    Chandra, R.P., Beatson, R.P. and Saddler, J.N. (2007) Treatment of Douglas-fir Heartwood TMP with Laccases: Effect of Treatment Conditions on Peroxide Bleaching -Journal of Wood Chemistry and Technology 27(2):73-82
    Ewanick, S., Bura, R. and Saddler, J.N. (2007) Acid-catalyzed steam pretreatment of Lodgepole pine and subsequent enzymatic hydrolysis and fermentation to ethanol. -Biotechnology and Bioengineering 98(4):737-746
    Adamowicz, V., Boyda, A. and McFarlane, P.N. (2007): (2007) Global Wood Supply. Report #02 in the Series on “Drivers of Change in Canada’s Forests and Forest Sector”, prepared for the Forest Futures Project of the SFM Network, University of Alberta. 16 pp. November. -SFM Network, University of Alberta
    Mabee, W.E., Fraser, E.D.G., McFarlane, P.N. and Saddler, J.N. (2007) Canadian biomass reserves for biorefining. -Applied Biochemistry and Biotechnology 129 (1-3) 22-40.
    Tu, M., Chandra, R.P. and Saddler, J.N. (2007) Recycling Cellulases during the Hydrolysis of Steam Exploded and Ethanol Pretreated Lodgepole Pine -Biotechnology Progress, 23(5) 1130-1137
    Berlin, A., Munoz, C., Gilkes, N., Massoumi Alamouti, S., Chung, P., Kang, K., Maximenko, V., Baeza, J., Freer, J., Mendoca, R. and Saddler, J.N. (2007) An Evaluation of British Columbian Beetle-Killed Hybrid Spruce for Bioethanol Productin --Applied Biochemistry and Biotechnology, 136-140:267-280
    Berlin, A., Maximenko, V., Gilkes, N. and Saddler, J.N. (2007) Optimization of Enzyme Complexes for Lignocellulose Hydrolysis -Biotechnology and Bioengineering, 97(2):287-296
    Ohgren, K., Bura, R., Lenicki, G., Saddler, J.N. and Zacchi, G. (2007) A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover -Process Biochemistry, 42(5):834-839
    Pan, X.-J., Xie, D., Yu, R., Lam, D. and Saddler, J.N. (2007) Pretreatment of lodgepole pine killed by mountain pine beetle using organosolv ethanol process: fractionation and process optimization -Industrial & Engineering Chemistry Research, 46(8):2609-2617
    Ohgren, K., Bura, R., Saddler, J.N. and Zacchi, G. (2006) Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. -Bioresource Technology 93(13):2503-2510
    Bjorheden, R., Domac, J., Mabee, W., Popescu, O., Richares, K., Richardson, J., Saddler, J.N., Smith, C.T. and Worgetter, M. (2006) Biofuels and Bioenergy: Challenges and Opportunities -Proceedings of a joint workshop of IEA Bioenergy Task 29, Task 31 and Task 39, August 2006, Vancouver, BC, Canada
    Pan, X Ehara K. Gilkes, N. Kadla, J. F. Saddler J. (2006) Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions and antioxidant activity J. Agr. Food Chem. 54, 5806-5813, 2006.
    Pan, X, Gilkes, N., Kadla, J., Pye, K., Saka, S., Ehara, K., Gregg, D., Xie, D., Lam, D. and Saddler, J. (2006) Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation Process: Optimization of process yields. Biotechnology and Bioengineering, published online, DOI:10.1002/bit.20905
    Berlin, A., Balakshin, M., Gilkes, N., Kadla, J., Maximenko, V, Kubo, S. and Saddler, J.N. (2006) Inhibition of cellulase, xylanase and ß-glucosidase activities by softwood lignin preparations Journal of Biotechnology 125(2):198-209
    Keating, J., Johansson, C., Saddler, J.N. and Beatson, R. (2006) The nature of chromophores in high extractive mechanical pulps: Western red cedar (Thuja plicata Donn) chemithermomechanical pulp (CTMP) Holzforschung 60:365-371
    Tu, M., Zhang, X., Kurabi, A., Gilkes, N., Mabee, W. and Saddler, J.N. (2006) Immobilization of ß-glucosidase on Eupergit C for lignocellulose hydrolysis Biotechnology Letters. 28: 151-156
    Pan, X.J., Gilkes, N. and Saddler, J. (2006) Effect of acetyl groups on enzymatic hydrolysis of cellulosic substates -Holzforschung, 94, 851-861
    Berlin, A., Maximenko, V., Bura, R., Kang, K., Gilkes, N. and Saddler, J. (2005) A rapid microassay to evaluate enzymatic hydrolysis of lignocellulosic subtrates Wiley InterScience, published online, DOI: 10.1002/bit.20783
    Berlin, A., Gilkes, N., Kurabi, A., Bura, R., Tu, M., Kilburn, D. and Saddler, J (2005) Weak lignin-binding enzymes - a novel approach to improve the activity of cellulases for hydrolysis of lignocellulosics Applied Biochemistry and Biotechnology, 121-124:163-170
    Mabee, W. and Saddler, J. (2005) Assessing the emerging biorefinery sector in Canada Applied Biiochemestry and Biotechnology 121-124:765-778
    Pan, X., Arato, C., Gilkes, N., Gregg, D.J., Mabee, W., Pye, E.K., Xiao, X. and Saddler, J.N. (2005) Biorefining of softwoods using ethanol organosolv pulping - preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. Biotechnology and Bioengineering. Vol. 90
    Pan, X., Xie, D., Gilkes, N., Gregg, D.J. and Saddler, J.N. (2005) Strategies to enhance the enzymatic hydrolysis of pretreated softwood with high residual lignin content Applied Biochemistry and Biotechnology. Vol. 121-124
    Berlin, A., Gilkes, N., Kilburn, D., Bura, R., Markov, A., Skomarovsky, A., Okunev, O., Gusakov, A., Maximenko, V., Gregg, D., Sinitsyn and Saddler, J.N. (2005) Evaluation of novel fungal cellulse preparations for ability to hydrolyze softwood substrates - evidence for the role of accessory enzymes -Enzyme and Microbial Technology 37(2005) 175-184
    (2004) It is not the enzyme

    (2004) Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates - evidence for the role of accesory enzymes

    Mabee, W.E., Gregg, D.J. and Saddler, J.N. (2004) International Energy Agency - Bioenergy. Current State of Fuel Ethanol Commercialization. Special Session B. Applied Biochemistry and Biotechnology. Vol 113-116
    Keating, J. D., Robinson, J., Cotta, M. A., Saddler, J. N., and Mansfield, S. D. (2004) An Ethanologenic Yeast Exhibiting Unusual Metabolism in the Fermentation of Lignocellulosic Hexose Sugars Industrial Microbiology and Biotechnology. 31(5):235-244
    Keating, J., Johansson, I., Saddler, J.N., and Beatson, R.P. (2004) Kinetics and Mechanism of Hydrogen Peroxide Brightening of Western Red Cedar CTMP Paper being presented at PAPTAC (Pulp and Paper Technical Association of Canada) Conference in Parksville, BC on April 16 and 17, 2004
    Saddler, J.N., Gregg, D.J. and Mabee, W.E. (2004) Progress in the Commercialization of Lignocellulosics-to-Ethanol Paper being presented at the 2nd World Conference and Technology Exhibition on Biomass for Energy, Industry and Climate Protection, May 2004.
    Pan, X., Zhang, X., Gregg, D.J., and Saddler, J.N. (2004) Enhanced enzymatic hydrolysis of steam exploded Douglas-Fir wood by alkali-oxygen post-treatment. Applied Biochemistry and Biotechnology. Vol. 113-116. (Proceedings of the 25th Symposium on Biotechnology for Fuels and Chemicals, May 2003)
    Xiao, Z., Zhang, X., Gregg, D.J. and Saddler, J.N. (2004) Effects of sugar inhibition on cellulase and B-glucosidase during enzymatic hydrolysis of softwood substrates. Applied Biochemistry and Biotechnology. Vol 113 - 116.(Proceedings of the 25th Symposium on Biotechnology for Fuels and Chemicals, May 2003)
    Keating, J.D., Robinson, J., Bothast, R.J., Saddler, J.N. and Mansfield, S.D. (2004) Characterization of a Unique Ethanologenic Yeast Capable of Fermenting Galactose. Enzyme and Microbial Technology. 35(2/3):242-253
    Cullis, I.F., Saddler, J.N. and Mansfield, S.D. (2004) Effect of initial moisture content and chip size on the bioconversion efficiency of softwood lignocellulosics. Biotechnology and Bioengineering, 85(4):413-421.
    Stebbing, D.W., Zhang, X., Soong, G., Mansfield, S.D. and Saddler, J.N. (2004) Fungal enzyme treatment of newsprint mill white water – impact on water and fiber properties. Pulp and Paper Science, Vol. 30. No. 1:3-8
    O''Connor, D., Esteghlalian, A.R., Gregg, D.J. and Saddler, J.N. (2003) Carbon Balance of Ethanol from Wood: The effect of Feedstock Source in Canada. The Role of Boreal Forests and Forestry in the Global Carbon Budget. pp. 289-296 (Proceedings of the International Science Conference, Edm. Alta. May 2000)
    Bura, R., Bothast, R.J., Mansfield, S.D. and Saddler, J.N. (2003) Optimization of SO2-Catalysed Steam Pretreatment of Corn Fibre for Ethanol Production Applied Biochemistry and Biotechnology, Vol. 105-108: 319-335.
    Robinson, J., Keating, J.D., Mansfield, S.D. and Saddler, J.N. (2003) The fermentability of concentrated softwood derived hemicellulose fractions with and without supplemental cellulose hydrolysates. Enzyme and Microbial Technology, 33(6), 757-765.
    Mansfield, S.D. and Saddler, J.N. (2003) Application of Enzymes to Lignocellulosics American Chemical Society, Oxford University Press
    Graham, P.J., Gregg, D.J. and Saddler, J.N. (2003) Wood-Ethanol for Climate Change Mitigation in Canada Applied Biochemistry and Biotechnology Vol. 105-108. (Proceedings of the 24th Symposium on Biotechnology for Fuels and Chemicals, May 2002)
    Himmel, M.D., Baker, J.O. and Saddler, J.N., Editors (2003) Glycosyl Hydrolases for Biomass Conversion ACS Symposium Series 769
    Saddler, J.N., R.A. Kozak and S.B. Watts (2002) A University Perspective on Innovations in Forestry Science and Technology. The Forestry Chronicle 78(1):115-116.
    Zhang, X., Eigendorf, G., Stebbing, D.W., Mansfield, S.D. and Saddler, J.N. (2002) Degradation of trilinolein by laccase enzymes. Archives of Biochemistry and Biophysics. 405(1):44-54.
    Esteghlalian, A.R., Mansfield, S.D. and Saddler, J.N. (2002) Cellulases: Agents for Fiber Modification or Bioconversion? The effect of substrate accessibility on cellulose enzymatic hydrolyzability. L. Viikari & R. Lantto (Eds.), Biotechnology Progress, Vol. 21, pp. 21-36. Elsevier Science.
    Esteghlalian, A., Graham, P., Gregg, D.J., O''''Connor, D. and Saddler, J.N. (2002) The Potential of Short Rotation Forestry on Marginal Farmland in BC and Alberta to Provide a Feedstock for Energy Generation and to Reduce Greenhouse Gas Emissions Suststainable Forest Management Network (Final project report 2002-5)
    Mais, U., Esteghlalian, A.R. and Saddler, J.N. (2002) Influence of Mixing Regime on Enzymatic Saccharification of Steam-Exploded Softwood Chips. Applied Biochemistry and Biotechnology, Vol 98-100, 463-472. (Proceedings of the 23rd Symposium on Biotechnology for Fuels and Chemicals, Colorado, May 2001)
    Robinson, J., Keating, J., Boussaid, A., Mansfield, S.D. and Saddler, J.N. (2002) The influence of bark on the fermenation of douglas-fir whitewood pre-hydrolysates Applied Microbiology and Biotechnology. 59 (4-5): 443-448
    Bura, R., Mansfield, S.D., Saddler, J.N. and Bothast, R.J. (2002) SO2-catalysed steam explosion of corn fibre for ethanol production. Applied Biochemistry and Biotechnology 98(100):59-72.
    Lu, Y., Yang, B, Gregg, D.J., Saddler, J.N. and Mansfield, S.D. (2002) Cellulase adsorption and an evaluation of enzyme recycle during the hydrolysis of steam-exploded softwood residues. Applied Biochemistry and Biotechnology, Vol. 98-100, 641-654.
    Mais, U., Esteghlalian, A.R., Saddler, J.N. and Mansfield, S.D. (2002) Enhancing the enzymatic hydrolysis of cellulosic materials using simultaneous ball milling. Applied Biochemistry and BiotechnologyVol 98-100, 815-832.
    Yang, B., Boussaid, A., Mansfield, S.D., Gregg, D.J. and Saddler, J.N. (2002) A fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam exploded softwood substrates. Biotechology and Bioengineering 77(6):678-684.
    Zhang, X., Stebbing, D.W., Soong, G, Saddler, J.N. and Beatson, R.P. (2002) A combined fungal and enzyme treatment system to remove TMP/newsprint mill white waters substances. TAPPI 1(2):21-27.
    Boussaid, A., Cai Y., Robinson, J., Gregg, D.J., Nguyen, Q. and Saddler, J.N. (2001) Sugar recovery and fermentability of hemicellulose hydrolysates from steam-exploded softwoods containing bark. Biotechnology Progress 17(5):887-892.
    Ingesson, H., Zacchi, G., Yang, B., Esteghlalian, A.R. and Saddler, J.N. (2001) The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose. Biotechnology 88:177-182.
    Esteghlalian, A.R., Bilodeau, M., Mansfield, S.D.and Saddler, J.N.. (2001) Do enzymatic digestibility and Simons' stain reflect the differences in the available surface area of lignocellulosic substrates. Biotechnology Progress 17:1049-1054.
    Esteghlalian, A.R., Srivastava, V., Gilkes, N.R., Kilburn, D.G., Warren, R.A.J. and Saddler, J.N. (2001) Do Cellulose Binding Domains Increase Substrate Accessibility? Applied Biochemistry and Biotechnology, Vol. 91-93, 575-592 (Proceedings of the 22nd Symposium on Biotechnology for Fuels and Chemicals, Tenessee, May 2000)
    S.M. Shevchenko, L. Pazner, J.N. Saddler (2001) Treatment of Organosolov and Steam Explosion Lignins with Molecular Hydrogen Iodide: A Structural Probe Cellulose Chemistry and Technology. Vol 35, 3-4, 295-318
    S.M. Shevchenko, K. Chang, D. G. Dick, D.J. Gregg and J.N. Saddler (2001) Structure and Properties of Lignin in Softwoods after SO2 - Catalyzed Steam Explosion and Enzymatic Hydrolysis Cellulose Chemistry and Techonology. Vol. 35, 5-6, 487-502
    Saddler, J.N., Zhang, X., Beatson, R.P., Stebbing, D.W., Cai, Y., Soong, JJ. (2001) Enzymatic and Microbial Treatment of Concentrated and Recycled Pulp Effluents Sustainable Forest Management Network (Final project report 2001-21)
    Johannsson, C.I., Saddler, J.N. and Beatson, R.P. (2000) Characterization of the Polyphenolics Related to the Colour of Western Red Cedar (Thuja plicata Donn) Heartwood Holzforschung 54(3): 246-254
    Johansson, I., Beatson, R.P. and Saddler, J.N. (2000) Fate and Influence of Western Red Cedar Extractivesin Mechanical Pulping. Wood and Fibre Science 34(5): 389-401
    Shevchenko, S., Paszner, L. and Saddler, J.N. (2000) Structural Changes in Lignin During Organosolv Pulping and Steam Explosion as Evidenced by Mild Depolymerization with Molecular Hydrogen Iodide. In press. Cellulose Chemistry and Technology 35 (5/6)
    Zhang, X., Stebbing, D.W., Saddler, J.N., Beatson, R.P. and Kruus, K. (2000) Enzyme Treatments of the Dissolved and Colloidial Substances Present in Mill White Water and the Effects on the Resulting Paper Properties. Journal of Pulp and Paper Science. 3(20) pp. 321-335
    Saddler, J.N. (2000) The Potential of Western Red Cedar as a Furnish for High Quality Mechanical Pulps. Wood and Fibre Science
    Esteghlalian, A., Boussaid, A., Gregg, D.J., Lee, K.H., Saddler, J.N., (2000) Steam Pretreatment of Douglas-Fir Wood Chips: Can Conditions for Optimum Hemicellulose Recovery Still Privide Adequate Access for Efficient Enzymatic Hydrolysis? Applied Biochemistry and Biotechnology (Proceedings of the 21st Symposium on Biotechnology for Fuels and Chemicals, Colorado, May 1999)
    Esteghlalian, A., Srivastava, V., Gilkes, N., Gregg, D.J.and Saddler, J.N. (2000) An Overview of Factors Influencing the Enzymatic Hydrolysis of Lignocellulosic Feedstocks. ACS Series X, Ed. M. Himmel, J. Baker and J.N. Saddler.
    Esteghlalian, A.R., Srivastava, V., Gilkes, N.R., Kilburn, D.G., Warren, R.A.J. and Saddler, J.N. (2000) Interaction Between Cellulose and a Cellulose-Binding Domain (CBDCex) From Bacterium Cellulomonas fimi and Its Effect on Substrate Enzymatic Susceptibility. NREL Proceedings of the Twenty-second Symposium on Biotechnology for Fuels and Chemicals.
    Shevchenko, S.M., Chang, K., Robinson, J. and Saddler, J.N. (2000) Optimization of monosaccharide recovery by post-hydrolysis of the water-soluble hemicellulose component after steam explosion of softwood chips. Bioresource Technology 72(3): 207-211.
    Serreqi, A., Gamboa, H., Stark, K., Saddler, J.N. and Breuil, C.. (2000) Resin acid markers for total resin acid content in-mill process lines of a TMP/CTMP pulp mill. Water Research 34(5):1727-1733.
    Wu, M.M., Chang, K., Gregg, D.J., Boussaid, A., Beatson, R. P. and Saddler, J.N. (1999) Optimization of steam explosion to enhance hemicellulose recovery and enzymatic hydrolysis of softwoods. Applied Biochemistry and Biotechnology. Vol. 77-79: 47-54. (Proceedings of the 20th Symposium on Biotechnology for Fuels and Chemicals, Tennessee, May, 1998)
    Zhang, X., Beatson, R.P., Cai, Y.J. and Saddler, J.N. (1999) Accumulation of specific dissolved and colloidal substances during white water recycling affects paper properties. Pulp and Paper Science. Vol. 25(6):206-210.
    Beatson, R.P., X. Zhang, D. Stebbing, and J. Saddler. (1999) The dissolved and colloidal fractions of white water: Impact of paper properties and degradation by enzymes. -Vol. 1:200-203 in 10th Int'l Symp. on Wood and Pulp Chemistry, Yokohama, Japan.
    Boussaid, A. and Saddler, J.N.. (1999) Adsorption and activity profiles of cellulases during the hydrolysis of two Douglas-fir pulps. Enzyme and Microbiology Technology. 24 (3-4):138-143.
    Boussaid, A., Robinson, J., Cai, Y.J., Gregg, D.J. and Saddler, J.N. (1999) Fermentability of the hemicellulose-derived sugars from steam-exploded softwood (Douglas-fir). Biotechnology and Bioengineering 64(3): 284-289
    Chandra, R.P., Beatson, R.P., de Jong, E. and Saddler, J.N. (1999) The Effects of Treatment with the White Rot Fungus Trametes Versicolor and Laccase Enzymes on the Brightness of Douglas-Fir Heartwood Derived Thermomechanical Pulps. Wood Chemistry and Technology. 19(1-2): 61-78.
    Lumme, M.S., Mansfield, S.D. and Saddler, J.N. (1999) The effects of hydrolytic enzyme treatments on three British Columbian interior fir kraft pulps differing in their initial fiber coarseness. Wood and Fiber Science. 31(4): 385-393.
    Mansfield, S.D., Mooney, C. and Saddler, J.N. (1999) Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnology Progress. 15(5): 804-816.
    Ramos, L.P., Filho, A.Z., Deschamps, F.C. and Saddler, J.N.. (1999) The effect of trichoderma cellulases on the fine structure of a bleached softwood kraft pulp. Enzyme and Microbiology Technology. 24:371-380.
    Saddler, J.N., X. Zhang, D. Stebbing, R.P. Beatson, B.G. Chalmers, and J.T. Ling. (1999) The different effects of white water dissolved and colloidal fractions on paper properties and effects of enzyme treatments on their removal. PACWEST, Whistler. pp. 18-24.
    Shevchenko, S.M., Beatson, R.P. and Saddler, J.N. (1999) The nature of lignin from steam explosion/enzymatic hydrolysis of softwood: Structural features and possible uses. Applied Biochemistry and Biotechnology. Vol. 77-79: 867-876. (Proceedings of the 20th Symposium on Biotechnology for Fuels and Chemicals, Tennessee, May 1998)
    Mansfield, S.D. and Saddler, J.N. (1999) Sheet properties of Douglas-fir kraft pulp after selective treatments of different fibre length fractions with cellulases. Pulp and Paper Science 25(3): 84-89.
    Mansfield, S.D., Swanson, D.J., Roberts, N., Olson, J.A. and Saddler, J.N. (1999) Enhancing Douglas-fir pulp properties with a combination of enzyme treatments and fiber fractionation. Tappi. 82(5):152-158.
    Mooney, C.A., Mansfield, S.D., Beatson, R.P. and Saddler, J.N. (1999) The effect of fiber characteristics on hydrolysis and cellulase accessibility to softwood substrates. Enzyme Microbilogy Technology. 25(8-9): 644-650.
    Gübitz, G.M., C.D. Edgar, S.D. Mansfield, J.N. Saddler, and W. Steiner. (1999) Role of cellulase/hemicellulase synergism in the modification of pretreated wood substrates. Int'l Symp. Biotechnol. Lignocell. Mat. Curitiba, Brazil. pp. 45-56.
    Lee, D., A.H.C. Yu and J.N. Saddler. 1996 (1999) Effect of lignin on cellulose recycling strategies. pp. 547-550 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research. Proc. 6th Int'l Conf. on Biotechnology in the Pulp and Paper Industry.
    Gübitz, G.M., S.D. Mansfield and J.N. Saddler. (1998) Effectiveness of two endoglucanases from Gloeophyllum sp. in deinking mixed office waste. 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 3:135-138.
    Lumme, M.S., S.D. Mansfield, and J.N. Saddler. (1998) Effect of enzyme treatment on two British Columbia interior fir kraft pulps. 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 3:163-166.
    Mansfield, S.D., A.R. Dickson, and J.N. Saddler. (1998) Improving paper properties by a selective enzymatic treatment of coarse pulp fibres. 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 1:189-192.
    Mansfield, S.D., B. Saake, G.M. Gübitz, E. de Jong, J. Puls, and J.N. Saddler. (1998) Identification, purification and characterisation of the predominant endoglucanases from two brown-rot fungal strains of Gloeophytllum. pp. 227-244 in Proc. 1997 Tricel Symp. Ghent, Belgium.
    Mooney, C.A., S.D. Mansfield, R.P. Beatson, and J.N. Saddler. (1998) The effect of fibre characteristics on accessibility and hydrolysis of softwood substrates. 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 3:99-102.
    Mooney, C.A., S.D. Mansfield, M.G. Touhy, and J.N. Saddler. (1998) The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods. Bioresource Technol. 64(2):113-119.
    Stalbrand, H., S.D. Mansfield, J.N. Saddler, D.G. Kilburn, R. Anthony J. Warren, and N.R. Gilkes. (1998) Analysis of molecular size distributions of cellulose during hydrolysis of cellulose by recombinent cellulomonas fimi Beta-1, 4-glucanases. App. Environ. Microbiol. 64(7):2374-2379.
    Edgar, C.D., S.D Mansfield, G.M. Gübitz, and J.N. Saddler. (1998) The synergistic effects of endoglucanase and xylanase in modifying Douglas-fir kraft pulp. pp.75-87 in Eriksson, K.-E. L and Cavaco-Paulo, A.M. (eds.), Enzyme Applications in Fibre Processing., Oxford University Press.
    Gregg, D.J., A. Boussaid and J.N. Saddler. (1998) Technoeconomic evaluations of a generic wood-to-ethanol process: Process effect of increased cellulose yields and enzyme recycle. Bioresource Technol. 63(1998):7-12.
    Gubitz, G.M., D.W. Stebbing, C.I. Johansson, and J.N. Saddler. (1998) Lignin-hemicellulose-complexes restrict enzymatic solubilisation of mannan and xylan from dissolving pulp. Appl. Microbiol. Biotechnol. 50:390-395.
    Gubitz, G.M., S.D. Mansfield, D. Bohm, and J.N. Saddler. (1998) Effect of endoglucanases and hemicellulases in magnetic and flotation deinking of xerographic and laser-printed papers. Journal of Biotechnology 65:209-215.
    Kyriacou, P., E. de Jong, C. Johansson, R.P. Chandra, and J.N. Saddler. (1998) Bleaching of western red cedar and Douglas-fir mechanical pulps. TAPPI J. 81(6):188-195.
    Mansfield, S.D., J.N. Saddler and G.M. Gubitz. (1998) Characterisation of two endoglucanases from the brown-rot fungi Gloeophyllum sepiarium and Gloeophyllum trabeum. Enzyme Microb. Technol. 23:133-140.
    Ryan, S.E., E. de Jong, G.M. Gubitz, M. Tuohy, and J.N. Saddler. (1998) The potential of hydrolytic enzymes to modify Douglas-fir derived bleached mechanical pulps. pp. 214-226 in Claeyssens, M. et al. (eds.), Carbohydrases from T. reesei and other microorganisms., The Royal Society of Chemistry.
    Gregg, D.J. and J.N. Saddler. . (1997) A review of techno-economic modelling methodology for a wood-to-ethanol process. In Proc. 18th Symp. Biotechnology for Fuels and Chemicals. Appl. Biochem. Biotechnol. 63/65:609-623
    Liss, S.N., P.A. Bicho, P.N. McFarlane, and J.N. Saddler. (1997) Microbiology and biodegradation of resin acids in pulp mill effluents: A mini review. Can. J. Microbiol. 75:599-611.
    Wong, K.K.Y., L.A. Martin, F.M. Gama, J.N. Saddler, and E. de Jong. (1997) Bleach boosting and direct brightening by multiple xylanase treatments during peroxide bleaching of Kraft pulps. Biotechnol. Bioengin. 54:312-318.
    de Jong, E., K.K.Y. Wong, and J.N. Saddler. (1997) The mechanism of xylanase prebleaching of kraft pulp - an examination using model pulps prepared by depositing lignin and xylan on cellulose fibers. Holzforschung 51:19-26.
    Zhang, Y., P.A. Bicho, C. Breuil, J.N. Saddler, and S.N. Liss. (1997) Resin acid degradation by bacterial strains grown on CTMP effluent. Water Sci. Tech. 35:33-39.
    Gregg, D.J. and J.N. Saddler. (1997) The IEA network: Biotechnology for the conversion of lignocellulosics. pp. 55-60 in Proc. 2nd European Motor Biofuels Forum, Graz, Austria.
    Saddler, J.N. and D.J. Gregg. (1997) Steam explosion and bioconversion of softwoods. pp. 135-138 in M. Stumborg (ed.), Proc. '97 Ethanol Research and Development Workshop, Ottawa.
    Mansfield, S.D. and J.N. Saddler. (1997) The use of enzymes to enhance Douglas-fir pulp characteristics. 1997 TAPPI Biological Sciences Symposium. October 19-23, 1997. San Francisco, CA. TAPPI Press 1:279-282.
    Mansfield, S.D., E. de Jong and J.N. Saddler. (1997) Cellobiose dehydrogenase, an active agent in cellulose depolymerization. App. Environ. Microbiol. 63(10): 3804-3809.
    Mansfield, S.D., E. de Jong and J.N. Saddler. (1997) The potential of enzymes to selectively modify pulp characteristics. In Proc. '97 Spring Conf. of CPPA Pacific Coast and Western Branches, Whistler Paper 3B:1-4.
    Mansfield, S.D., E. de Jong, R.S. Stephens, and J.N. Saddler. (1997) Physical characterization of enzymatically modified kraft pulp fibres. J. Biotechnol. 57(1-3): 205-216.
    Mooney, C.A., S.D. Mansfield, M.G. Touhy, and J.N. Saddler. (1997) The effect of lignin content on cellulose accessibility and enzymatic hydrolysis of softwood pulps. 1997 TAPPI Biological Sciences Symposium. October 19-23, 1997. San Francisco, CA. TAPPI Press 1:259-265.
    Li, K., A. Serreqi, C. Breuil and J.N. Saddler. (1997) Quantification of resin acids in CTMP effluents using an enzyme-linked immunosorbent assay. Water Science & Technol. 35(2-3):93-99.
    Mansfield, S.D., K.K.Y. Wong, E. de Jong, and J.N. Saddler. (1996) Xylanase prebleaching of different fiber length fractions of Douglas-fir kraft pulp. Appl. Microbiol. & Biotechnol. 46:319-326.
    de Jong, E., K.K.Y. Wong, L.A. Martin, S.D. Mansfield, F.M. Gama, and J.N. Saddler. (1996) Molecular mass distribution of materials solubilized by xylanase treatment of Douglas-fir kraft pulp. pp. 44-62 in T. Jeffries (ed.), Enzymes for Pulp and Paper Processing., American Chemical Society (ACS) Symp. Series No. 655.
    Mansfield, S.D., K.K.Y. Wong, T. Astle, and J.N. Saddler. (1996) Enzymatic modification of coarse pulp fibers derived from Douglas-fir. pp. 173-176 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research. Proc. 6th Int'l Conf. on Biotechnology in the Pulp and Paper Industry.
    Mansfield, S.D., K.K.Y. Wong, E. de Jong, and J.N. Saddler. (1996) Modification of Douglas-fir mechanical and kraft pulps by enzymatic treatment. Tappi J. 79(8):125-132.
    Werker, A.G., P.A. Bicho, J.N. Saddler, and E.R. Hall. (1996) Surface tension changes related to the biotransformation of dehydro-abietic acid by Mortierella isabellina. pp. 139-150 in M.R. Servos (ed.), Environmental Fate and Effects of Pulp and Paper Mill Effluents. St. Lucie Press.
    Wong, K.K.Y., E. de Jong, and J.N. Saddler. (1996) Size-exclusion chromatography of lignin- and carbohydrate-containing samples using alkaline effluents. J. Chromatography A 737:193-203.
    Wong, K.K.Y., E. de Jong, and J.N. Saddler. (1996) Lignin-xylan complexes in kraft pulp appear to be target substrates for xylanase prebleaching. pp. 93-98 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research., Proc. 6th Int'l Conf. on Biotechnology in the Pulp and Paper Industry.
    Wong, K.K.Y., S.L. Nelson, and J.N. Saddler. (1996) Xylanase treatment for the peroxide bleaching of oxygen delignified kraft pulps derived from three softwood species. J. Biotechnol. 48:137-145.
    Wong, K.K.Y., S. Yokota, J.N. Saddler, and E. de Jong. (1996) Enzymatic hydrolysis of lignin-carbohydrate complexes isolated from kraft pulps. J. Wood Chem. Technol. 16(2): 121-138.
    Bicho, P.A., T. Chen, S. Avramidis, R.L. Zwick, C. Breuil, and J.N. Saddler. (1996) Characterization and treatment of condensates generated from radio-frequency/vacuum dried softwoods. For. Prod. J. 46(10):51-56.
    Li, K., T. Chen, P. Bicho, C. Breuil, and J.N. Saddler. (1996) Factors affecting gas chromatographic analysis of resin acids present in pulp mill effluents. Toxicological and Environm. Chem. 57:1-16.
    Li, K., T. Chen, P. Bicho, C. Breuil, and J.N. Saddler. (1996) A comparison of gas chromatographic and inununochemical methods for quantifying resin acids. pp. 119-128 in Servos el al. (eds.), Environmental Fate and Effects of Pulp and Paper Mill Effluents., St. Lucie Press.
    de Jong, E., K.K.Y., L.R. Windsor, and J.N. Saddler. (1996) Xylanase prebleaching of model pulp. pp. 127-130 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research., Proc. 6th Int'l Conf. on Biotechnology in the Pulp and Paper Industry.
    Gregg, D. and J.N. Saddler. (1996) Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood-to-ethanol process. Biotech/Bioeng. 51:375-383.
    Gregg, D. and J.N. Saddler. (1996) A technoeconomic assessment of the pretreatment and fractionation steps of a biomass-to-ethanol process. Appl. Biochem. Biotechnol. 57/58:711-727.
    Bicho, P.A., V.A. Martin and J.N. Saddler. (1996) Resin acid biodegradation by bacteria isolated from bleached kraft mill effluent: Specificity and induction. pp. 611-616 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research. Proc. 6th Int'l Conf. on Biotechnology in the Pulp and Paper Industry.
    Bullock, C.M., P.A. Bicho, and J.N. Saddler. (1996) The influence of the high and low molecular weight fractions on the microbial activity of activated sludge. Water Research 30(5):1095-1102.
    Bullock, C.M., P.A. Bicho, Y. Zhang, and J.N. Saddler. (1996) A solid chemical oxygen demand (COD) method for determining biomass in waste waters. Water Research 30(5):1280-1284.
    Nelson, S.N., K.K.Y. Wong, R.P. Beatson, and J.N. Saddler. (1995) The use of xylanase for peroxide bleaching of kraft pulps derived from different softwood species. Pulp and Paper Canada 96(7):42-45.
    Nelson, S., K.K.Y. Wong, R.P. Beatson, and J.N. Saddler. (1995) Xylanase prebleaching of brownstock and oxygen delignified kraft pulp derived from Douglas-fir. pp. 413-418 in Proc. IWC Meeting, Helsinki.
    Werker, A.G., P.A. Bicho, J.N. Saddler, and E.R. Hall. (1995) Surface tension changes related to the biotransformation of dehydroabietic acid by Mortierella isabellina. pp. 134-137 in Servos et al. (eds.), Environmental Fate and Effects of Pulp and Paper Mill Effluents. St. Lucie Press.
    Yokotu, S., K.K.Y. Wong, J.N. Saddler, and I.D. Reid. (1995) Molecular weight distribution of xylan/lignin mixtures from kraft pulps. Pulp & Paper Canada 96(4):39-41.
    Yu, A.H.C. and J.N. Saddler (1995) Identification of essential cellulase components in the hydrolysis of a steam-exploded substrate Biotechnol. Appl. Biochem. 21:185-202.
    Yu, A.H.C., D. Lee and J.N. Saddler (1995) Adsorption and desorption of cellulase components during hydrolysis of a steamexploded birch substrate. Biotechnol. Appl. Biochem. 21:203-216.
    Bicho, P.A., V. Martin, and J.N. Saddler. (1995) Growth, induction and substrate specificity of dehydroabietic acid degrading bacteria isolated from a kraft mill effluent enrichment. Appl. Environ. Microbiol. 61(9):3245-4350.
    Gregg, D. and J.N. Saddler. (1995) Bioconversion of lignocellulosic residues to ethanol: process flowsheet development. Biomass and Bioenergy 9:287-302.
    Li, K., M. Chester, J. Kutney, C. Breuil, and J.N. Saddler. (1995) Development of an immunoassay for the detection of resin acids in pulp mill effluents. pp. 675-688 in M. Moo-Young, W.A. Anderson, A.M. Chakrabarty (eds.), Environmental Biotechnology: Principles and Practice., Kluwer Academic Publishers.
    Saddler, J.N. and Penner, M.H. Editors (1995) Enzymatic Degradation on Insoluble Carbohydrates ACS Symposium Series 618. (Proceedings from the 207th National Meeting of the American Chemical Society, California, March 1994)

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    Click for a large print-resolution photo of Jack (John) N Saddler
    Jack (John) N Saddler
    Forest Products Biotechnology

    B.Sc. (Hons) (1975) Edin., Ph.D (1978) Glas.

    Contact Info:
     Faculty of Forestry, Office of The Dean, 2nd Floor, Forest Sciences Centre 2004-2424 Main Mall Vancouver, B.C. Canada V6T 1Z4
    Phone: 604-822-9741
    Fax: 604-822-8157
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